Clinical significance of miR-143 and miR-145 expression in gastric gastrointestinal stromal tumor
10.3760/cma.j.issn.1007-631X.2010.08.025
- VernacularTitle:miR-143和miR-145在胃间质瘤组织中的表达及其意义
- Author:
Zhi LIU
;
Shaoliang HAN
;
Yingpeng HUANG
;
Wenyi WU
;
Junlin LI
;
Xiangyang XUE
;
Xian SHEN
- Publication Type:Journal Article
- Keywords:
Gastrointestinal stromal tumors;
microRNAs;
Pathology,clinical
- From:
Chinese Journal of General Surgery
2010;25(8):678-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of miR-143, miR-145 in the development of gastric gastrointestinal stromal tumor. Methods The expression levels of miR-143 and miR-145 in 21 cases of gastric gastrointestinal stromal tumor and the matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-PCR, and its correlation with clinicopathologic features of gastric gastrointestinal stromal tumor were analyzed. Results Expression level of miR-145 were significantly higher in tumor than adjacent normal tissues (P＜0.01 ) and that with mitotic count ≥ 5/50HPF cases was significantly lower than that with mitotic count ＜5/50HPF cases (P=0.02). miR-145 expression in huge tumor (＞10 cm)was significantly lower than that in the large tumor (5～10 cm) and small tumor (2～5 cm) (P=0.048).By Fletcher risk stratification system, miR-145 expression in high-risk cases was significantly lower than that in the intermediate-risk and low-risk cases (P=0.048). While the expression of miR-145 in low-risk group was significantly different compared to that in intermediate-risk group and high-risk group (P=0.01).There was no difference between the expressions of miR-143 in tumor and that in normal tissue(P=0.06).Conclusion In gastric gastrointestinal stromal tumor, MiR-145 expression is significantly higher in tumor than adjacent normal tissues. miR-145 is closely associated with tumor size. mitotic counts and Fletcher risk stratification system.
