Study on biological characteristics of adult adipose-derived mesenchymal stem cells and its differentiation into cardiomyocytes in vitro

VernacularTitle:体外诱导成人脂肪干细胞向心肌细胞分化的研究
Author: Gang REN ; Yunfei BIAN ; Xiaojia WU ; Maolian LI ; Chuanshi XIAO
Publication Type:Journal Article
Keywords: Adipocytes/PH; Stem cells/PH; Cell differentiation; Myocytes,cardiac/PH
From: Journal of Chinese Physician 2010;12(7):907-909
CountryChina
Language:Chinese
Abstract: Objective To induce adipose-derived mesenchymal stem cells (ADMSCs) differentiation into cardiomyocytes, so as to provide stem cells for myocardial regeneration.Method ADMSCs were isolated and cultured from adult adipose tissue in vitro.They were induced with 10μmol/L 5-azacytidine (5Aza) for 24h.At the 7th, 14th, 21st days after induction, the expression of α-sarcomeric actin, and myosin heavy chain were repeatedly detected by immunocytochemistry.The expression of ANP was detected by RT-PCR.Results At the 7th day after induction, there was no expression of α-sarcomeric actin and MHC.At the 14th day, there was a little expression of α-sarcomeric actin and MHC that could be seen in cells.At the 21st day, there was increased expression of α-sarcomeric and MHC, and the expression of ANP was positive results detected by RT-PCR.Conclusions 5-Aza can induced ADMSCs to differentiate into cardiomyocytes.ADMSCs might be a potential source of cell transplantation for myocardial.