Effect of dendritic cells transfected with total RNA of human colon cancer Lovo cells on specific cytotoxicity of cytokine-induced killer cells in vitro
10.3760/cma.j.issn.1673-422X.2010.06.023
- VernacularTitle:负载人结肠癌Lovo细胞总RNA抗原树突状细胞对细胞因子诱导的杀伤细胞在体外特异性杀伤的影响
- Author:
Jifang LIU
;
Yanchao QI
;
Bo YANG
;
Minying LU
;
Dongxiao PANG
;
Hongzhuo SHEN
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
Killer cells;
Antigens
- From:
Journal of International Oncology
2010;37(6):477-480
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of dendritic cells (DC) primed by total RNA extracted from human colon cancer Lovo cell on specific cytotoxicity of cytokine-induced killer cells (CIK) in vitro.Methods Cord blood mononuclear cells extracted by Ficoll density gradient centrifuge were induced into CIK and DC cells separately, and their Immunophenotype was detected by Flow cytometer. Trizol harvested total RNA from colon cancer cell Lovo and the RNAs were loaded to DCs obtained from cord blood as tumor anti gens. Effectors were grouped accordingly as CIK cells co-cultured with DCs transfected with Lovo RNA, CIK cells co- cultured with unloaded DCs and CIK cells. Targets was Lovo cells. In vitro cytotoxicity of CHK cells was extured with DCs loaded with Lovo RNA(76.49%±4.21%), DC + CIK group was lower(53.84% ± 2.15%),and CIK cells group possessed the lowest cytotoxicity(32.20% ± 3.07%), showing statistic significance( P ＜0.05). Conclusion Extraction of total RNA from tumor cells is simple and easy for clinical implementation.Total RNAs acted as antigen to pulse DCs can strengthen the specific cytotoxicity of CIK cells, which will have good prospects for clinical application.
