Effects of soybean isoflavone on action potential of guinea pig papillary muscle and sodium current in cultured neonatal rat cardiomyocyte
10.3760/cma.j.issn.0254-9026.2010.08.021
- VernacularTitle:大豆异黄酮对豚鼠乳头肌动作电位和心肌细胞钠电流的影响
- Author:
Lijuan MA
;
Wenjie ZHANG
;
Yuwei ZHANG
;
Chunyan ZHAO
- Publication Type:Journal Article
- Keywords:
Isoflavones;
Myocytes,cardiac;
Action potentials;
Soudium channels;
Guinea pigs
- From:
Chinese Journal of Geriatrics
2010;29(8):679-682
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of soybean isoflavone (SI) on the action potential of guinea pig papillary muscle and the sodium current in cultured neonatal rat cardiomyocyte.Methods The action potential of guinea pig papillary muscle was measured by glass suspension electrode and the sodium current in cultured neonatal rat cardiomyocyte was measured by whole cell patch-clamp technique. Results The SI reduced the action potential amplitude (APA) and shortened the 50% and 90% of duration of action potential (APD50 and APD90). The SI 0.1μg/ml inhibited sodium current in cardiomyocyte at a inhibition rate of 14.39 %, and the corresponding data of SI 1.0μg/ml at a inhibition rate of 23.48%. Conclusions The SI can reduce the APD of myocardiocyte and has a negative inotropic action on myocardium. These results indicate that SI inhibition of the sodium current may be one of mechanisms of its antiarrhythmic action.
