An in-vitro study of Lovastatin's biphasic effects on proliferation and adhesion of human umbilical vein endothelial cells
10.3760/cma.j.issn.1007-631X.2010.08.021
- VernacularTitle:洛伐他汀对人血管内皮细胞双向调节作用的实验研究
- Author:
Guojun ZHAI
;
Guoxiang DONG
;
Jun ZHAO
;
Weijuan YAO
- Publication Type:Journal Article
- Keywords:
Endothelial cells;
Lovastatin;
Cell proliferation;
Cell adhesion;
Umbilical veins
- From:
Chinese Journal of General Surgery
2010;25(8):665-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Lovastatin's effects on proliferation and adhesion of human umbilical vein endothelial cells (HUVECs). Methods Culture medium with different concentration of Lovastatin(0.001、0.01、0.1、1.0、10μmol/L) was prepared, HUVECs was cultured in 96 well-plate with the different medium. AT the point of 24,72 and 120 h, the cell's activity and quantity was assessed by MTT. HUVECs was cultured with Lovastatin in 6 well-plate for 24 hours, then collected the cells by trypsin digestion. The cells were seeded in 24 well-plate with 2×104/ml and adhering for 30 mins. Then counting the adhered cells in different wells. Results At 24 h, Lovastatin (0.01、 0.1 μmol/L ) promoted proliferation of HUVECs ( P ＜ 0.05 ); at 72 h, Lovastatin ( 1.0μmol/L) was positive accelerating cell growth(P＜ 0.05 ). While Lovastatin ( 10μmol/L) inhibited the proliferation significantly ( P ＜0.05 ) at 120 h. As HUVECs was cultured with Lovastatin for 24 hours, Lovastatin (0.1、1μmol/L) inhanced the adhesion capability of HUVECs significantly( P＜ 0.05 ). Conclusion Lovastatin had biphasic effects on proliferation and adhesion of HUVECs dependent on the concentration. Lovastatin (0.1、1.0 μmol/L) could promote the proliferation and adhesion, while at higher concentration ( 10.0μmol/L) it inhibits cell proliferation and adhesion.
