Ultrastructural observation and quantitative analysis of apoptosis of human gastric carcinoma cell SGC-7901 induced by Jiedu-Xiaozheng decoction
10.3760/cma.j.issn.1673-422X.2010.06.020
- VernacularTitle:解毒消癥饮诱导SGC-7901胃癌细胞凋亡的电镜观察和定量分析
- Author:
Ruhui LIN
;
Wenlie CHEN
;
Haixia HU
;
Liangpu ZHENG
;
Yunmei HUANG
;
Meiya HUANG
;
Gang CHEN
;
Jian DU
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Mitochondria;
Apoptosis
- From:
Journal of International Oncology
2010;37(6):465-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Jiedu-xiaozheng decoction on theUltrastructure changes and quantitative analysis of SGC-7901 in vitro and its mechanisms. Methods The compound prescription Jiedu-xiaozheng decoction was used to prepare serum containing drug. Serologic pharmacology methods was applied. The decoction was used to co-culture the gastric cells for24 h and 72 h, and the ultrastructure of tumor cells was observed by the transmission electron microscope. Then the parameters of the nucleus and mitochrondria were determined in SGC-7901 cells, including volume density(Vv) ,form factor PE( PE), average volume(V) ,length(L) average surrace area(S),contrasting to cytoplasma. The SIS powervision was applied to analyze their difference. Results Jiedu-xiaozheng decotion can result in reduced microvilli, deflated volume, decreased mitochondrion volume and reduced ridge. The volume density(Vv) ,form factor PE(PE) ,average volume(v) ,length (L) average surrace area(S) reduced in the 24 h group clearly in Jiedu-xiaozheng decoction group compared to control group. Conclusion Jiedu-xiaozheng decoction can significantly induce SGC-7901 cells apoptosis, exhibiting certain early ultrastructure changes such as marginated heterochromatin and mitochondrion pyknosis. The quantity analysis by electronic imaging system may objectively reveal the ultrastructural changes of cell nucleus and mitochondria.
