Preparation and evaluation of nanometer-scale bubbles with surfaces of N-palmitoyl chitosan
10.3760/cma.j.issn.1004-4477.2010.08.029
- VernacularTitle:聚糖纳米微泡超声造影剂的制备及其超声显影效果
- Author:
Yunbin XIAO
;
Jianguo BIN
;
Meiyu LI
;
Jiajia XIE
;
Juefei WU
;
Weilan WU
;
Yili LIU
;
Gangbiao JIANG
;
Jianping BIN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Nanostructures;
N-palmitoyl chitosan
- From:Chinese Journal of Ultrasonography
2010;19(8):719-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop nanometer-scale bubbles with surfaces of N-palmitoyl chitosan(PLCS) as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo. Methods The PLCS nanobubbles were prepared using a cutting technique at differential high-frequency of shear speed. Both optical and transmission electron micrography were performed to determine the nanobubble size and morphology. Concentration, size-distribution and zeta potential of the PLCS nanobubbles were measured by cell counting chamber, Malvern lazer particle analyzer and zeta-sizer at 1-day, 45-day and 90-day. The acoustic effects of the PLCS nanobubbles on myocardium and renal tissue in 6 normal rats were observed using bolus infusion of the nanobubbles intravenously. The maximum video intensity(VI) was measured.Results The PLCS nanobubbles with nice round-shape and uniform site-distribution were demonstrated.The mean diameter,concentration and zeta potential of the PLCS nanobubbles were (617 ± 12) nm, (7.2 ±0.6) × 109/ml and (52.9 ± 1.3)mV at the 1-day,and all of parameters did not change significantly in 45-day and 90-day ( P ＞ 0. 05). A significant contrast-enhancement was noted on myocardium and renal tissue during infusion of the nanobubbles. VI on both tissues was (15.6 ± 1.1)GU and (27.3 ± 2.5)GU,respectively. The visual contrast-enhancement last up to (10 ± 2)min. Conclusions The PLCS nanometerscale bubbles have excellent physical-features and contrast-enhanced ultrasound effects in vivo. It may develop as a novel contrast ultrasound agent which could cross endothelial cell membrances.
