Radiosensitization of microRNA-17-92 on human mantle cell lymphoma cells in vitro
10.3760/cma.j.issn.0254-5098.2010.04.007
- VernacularTitle:microRNA-17-92对人套细胞淋巴瘤细胞放射敏感性的影响
- Author:
Ping JIANG
;
Junjie WANG
;
Yong ZHAO
;
Enyu RAO
;
Na MENG
;
Zhenyou YOU
- Publication Type:Journal Article
- Keywords:
MicroRNA-17-92;
Mantle cell lymphoma;
Cell cycle;
Apoptosis;
Irradiation
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(4):403-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNA-17-92 on radiosensitivity of human mantle cell lymphoma cells. Methods Tetracycline-regulated pRevTet-On expression system was established to generate cell line Z138c-miR-17-92 with over-expressed miR-17-92 and cell line Z138c-TMP2. Cell proliferation was measured by 3 H-TdR incorporation and viable cell counting stained with typan blue. Cell cycle distribution was analysed by flow cytometry(FCM). Results More viable and proliferous cells were counted in group miR-17-92,when exposure dose was greater than 2 Gy and incubation time was longer than 48 h under the same condition (t = -3. 12 and -3.28,P ＜0. 05). The percentage of G2/M cells in group TMP2 was increased while no obvious cell cycle arrests were found in group miR-17-92 at 2 and 4 Gy (t = 2. 885, P ＜ 0.05 ). When cells were incubated for 96 h, higher percentage of propidium iodide (PI) positively stained cells were found in group TMP2 (24. 02% vs. 36. 16% )compared with group miR-17-92 (6.49% vs. 11.39% ) at 2 and 4 Gy, respectively( t = - 17.59, - 4. 972, P ＜ 0.05 ).Conclusions Overexpression of microRNA-17-92 decreased the radiosensitivity of human mantle cell lymphoma cells by inhibition of cell cycle changes and cell apoptosis.
