Effect of silencing Ku80 combined with γ-rays on growth of esophageal cancer xenografts

VernacularTitle:Ku80基因沉默联合γ射线对人食管癌细胞移植瘤生长的影响
Author: Qingshan YANG ; Yuanyuan LIU ; Feiyue FAN
Publication Type:Journal Article
Keywords: shRNA; Ku80; Esophageal cancer xenografs; Radiation
From: Chinese Journal of Radiological Medicine and Protection 2010;30(4):407-409
CountryChina
Language:Chinese
Abstract: Objective To investigate the inhibition effect of silencing Ku80 gene combined with irradiation on growth of esophageal cancer xenografs. Methods shRNA-Ku80 vector was constructed.The expression of Ku80 protein was inhibited by shRNA-Ku80 vector by using Western blotting. 20 BALB/c nude mice were randomly divided into 4 groups, including control group, radiation group, shRNA-Ku80 group and combined group. The growth of esophageal cancer xenografs was observed. The expression of Ku80 was examined in esophageal cancer xenografs by IHC. Results Effective target sequence was selected. The growth of esophageal cancer xenografs was inhibited by shRNA-H2 and radiation, especially in combined group. The inhibition rate of growth in three groups above was 32.0% , 39. 9% and 68. 9% ,respectively. The expression of Ku80 was reduced to 58% by shRNA-H2 in esophageal cancer xenografs ( t = 3.77, P ＜ 0. 05 ). Conclusions Combination of silencing Ku80 and radiation could enhance radiotherapeut effect of esophageal cancer xenografs.