Variation and significance of histamine receptors before and after the protection to the mucous membrane of urinary bladder of interstitial cystitis rat model
10.3760/cma.j.issn.1000-6702.2010.08.013
- VernacularTitle:间质性膀胱炎大鼠动物模型膀胱黏膜保护前后组胺受体变化及意义
- Author:
Ke LIU
;
Hui SHAN
;
Yong YANG
- Publication Type:Journal Article
- Keywords:
Cystitis,interstitial;
Receptors,histamine;
Hyaluronic acid;
Rats
- From:
Chinese Journal of Urology
2010;31(8):541-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of histamine receptors in bladder before and after the treatment by sodium hyaluronate in rats of interstial cystitis (IC). Methods Twenty IC model rats were randomly divided into experimental group and control group, with 10 for each group.The bladders in experimental group were filled with sodium hyaluronate, while the rats in control group were executed at once. The bladders were dyed with HE staining, special staining and immunohistochemistry staining to count the number of mononuclear cells and mast cells and observe the changes of histamine receptors. Results In experimental group,the counts of mononuclear cells and mast cells were 12.20±2.48 and 2.90±0.87 respectively;the numbers of average optical of histamine receptor H1, H2, H3, H4 were 0.015±0.007, 0.006±0.001, 0.007±0.004, 0.061±0.026 , respectively. In control group, the counts of mononuclear cells and mast cells were 23.90±3.07 and 7.08±1.23;the numbers of average optical of histamine receptor H1, H2, H3, H4 were 0.055±0.033, 0.031±0.023, 0.033±0.017, 0.091±0.059, respectively. The differences of mononuclear cells and mast cells were significant between the 2 groups(P＜0.01). The differences of average optical of histamine receptor H1, H2, H3 between the 2 group were significant (P＜0.05), while the difference of histamine receptor H4 was not significant(P＞0.05). Conclusion Histamine receptor H1, H2, H3 take part in the development of IC, the antagons may be used for the treament of IC.
