Expression of Survivin in T lymphocytes from allografts and its clinical significance
10.3760/cma.j.issn.0254-1785.2010.08.013
- VernacularTitle:凋亡抑制蛋白Survivin在移植物内T淋巴细胞中的表达及意义
- Author:
Bicheng CHEN
;
Hui GUO
;
Peng XIA
;
Yong CAI
;
Yirong YANG
;
Zhonghua CHEN
;
Sheng CHANG
- Publication Type:Journal Article
- Keywords:
Survivin;
Graft;
Lymphocytes;
Acute cellular rejection
- From:
Chinese Journal of Organ Transplantation
2010;31(8):492-495
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of survivin in T lymphocytes that were stimulated by Con A and alloantigens in renal grafts in vitro and in vivo. Methods According to the different treatments, the experiment was divided into three parts. (1) The C57BL/6 mice splenocytes stimulated by Con A (10 mg/L) were cultured in the RPMI-1640 medium. Following the proliferation blockade or not, the expression of Survivin in the splenocytes was detected. (2) The GVHR model was established by transfusing the C57BL/6 mice splenocytes into Balb/c× C57BL/6 F1 mice, and the expression of Survivin in the donor splenocytes was detected at the different time points. (3) Seventythree cases of clinical renal allograft biopsy specimens were collected, pathologically diagnosed and classified according to the Banff 97 classification, and then the expression of Survivin was detected.Results Survivin was expressed in the CD3+ splenocytes that received Con A stimulation. The positive cell count reached the peak on the day 3, and subsequently declined. In the GVHR model, the lymphocytes infiltration and Survivin expression were detected around the portal vein and portal area on the post-splenocytes-transfused day (PSTD) 4 to 12. But on the PSTD 14, the Survivin expression could not be detected in the infiltrated lymphocytes. In the renal allograft biopsy specimens,lymphocytes did not express Survivin in 13 specimens of the group without acute cellular rejection.was difference between the two groups (P＜0. 01 ). Conclusion The activated T cells possess the capacity to express Survivin, and the expression is time-dependent. For the characteristics of Survivin expression of T cells, it may be applied as an approach to diagnose the acute cellular rejection and judge its degree and stage in the clinical allograft biopsy specimens.
