Expression of ILT3 and ILT4 in dendritic cells of kidney transplantation recipients and its significance
10.3760/cma.j.issn.0254-1785.2010.08.009
- VernacularTitle:免疫球蛋白样转录物3和4在肾移植受者树突状细胞中表达的意义
- Author:
Lining JIA
;
Puxun TIAN
;
Wujun XUE
;
Xiaoming DING
;
Xiaoming PAN
;
Heli XIANG
;
Zhankui JIN
- Publication Type:Journal Article
- Keywords:
Immunoglobulin-like transcripts 3;
Immunoglobulin-like transcripts 4;
Kidney transplantation;
Immune hyporesponsiveness
- From:
Chinese Journal of Organ Transplantation
2010;31(8):478-480
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of immunoglobulin-like transcripts 3 (ⅡT3) and ILT4 in peripheral blood dendritic cells (DC) of kidney transplantation recipients and to analyze its significance in immunity hyporesponsiveness of transplantation. Methods Twenty kidney allograft recipients who were survived more than five years were recruited to two groups: renal function stable groups, chronic rejection groups, and 10 healthy volunteers served as a control group. The peripheral blood mononuclear cells (PBMC) were stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) and immature DC were obtained. The expression of ILT3 and ILT4 was detected by using flow cytometry. The level of HLA-G5 in serum was determined by using enzyme linked immunosorbent assay. Results ILT3 expression in renal function stable group was increased and decreased in chronic rejection groups as compared with control group (P＜0.05),but ILT4 expression had no significant difference among all groups. HLA-G5 in serum was significantly increased in renal function stable group as compared with other groups. Conclusion Expression of ILT3 and HLA-G was increased in the kidney transplantation recipients with stable renal function and long-term survival, suggesting that they may play an important role in inducing and maintaining periphery immune tolerance.
