Microencapsulated pancreatic B cell line as a bioartificial pancreas in agarose/PSSa microbcads
- VernacularTitle:琼脂糖胶/聚苯乙烯磺酸微囊化胰B细胞株MIN 6作为人工生物胰的实验研究
- Author:
Wenjing WANG
;
Zhuping WANG
- Publication Type:Journal Article
- Keywords:
MIN6;
Microencapsulation;
Agarose/PSSa
- From:
Chinese Journal of Organ Transplantation
1997;18(2):88-90
- CountryChina
- Language:Chinese
-
Abstract:
Presented in this study was the report of microencapsulated pancreatic B cell line MIN6 in agarose/polystyrene sulfonic acid (PSSa) microbeads as a bioartificial pancreas. One week after subcutaneous xenotransplantation to Lewis rats without use of any immunosup-pressants, the microbeads microencapsulated MIN6 cells were retrieved. The retrieved microbeads were perfectly intact, spherical, smooth and contained viable MIN6 cells. Fibrotic overgrowth and adhesions were rarely observed. The results of the static incubation study revealed that the secretion of insulin from the retrieved microbeads in response to a low glucose concentration was 19.20 ± 1.35μU·100 microbeads-1 /h ; In response to a high glucose stimulation, secretion of insulin was significantly and prominently increased to 51.47±3.82μU·100 microbeads-1/h. The results suggested that this agarose/PSSa microbeads microencapsulated MIN6 cells as a bioartificial pancreas possessed not only an excellent efficacy of immunoisolation, but also a good responsibility of insulin secretion to glucose stimulation.
