Effect of paclitaxel and gemcitabine on the expression of nucleosomal binding protein 1 in bladder cancer cell line T24
10.3760/cma.j.issn.1000-6702.2010.08.011
- VernacularTitle:紫杉醇和吉西他滨对膀胱癌细胞株T24中核小体结合蛋白1表达的影响及意义
- Author:
Cuijian ZHANG
;
Xuesong LI
;
Wahafu WASILIJIANG
;
Kun YAO
;
Gang SONG
;
Zhisong HE
;
Liqun ZHOU
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
HMGN1 protein;
Chemotherapy
- From:
Chinese Journal of Urology
2010;31(8):536-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of paclitaxel and gemcitabine on the expression of nucleosomal binding protein 1(NSBP1) in bladder cancer cell line T24 and its significance. Methods Reverse transcription PCR(RT-PCR) and western blotting were used to detect the expression of NSBP1 in bladder cancer cell T24 treated by different concentration(10% ,40% ,80% IC50) of paclitaxel and gemcitabine for 48 hours. Results RT-PCR showed that relative optical density (OD) of NSBP1 in bladder cancer cell T24 treated by 0%, 10%,40% and 80% IC50 paclitaxel were 0.392 ±0.024, 0.227±0.037, 0.135±0. 063 and 0.091 ±0.017, respectively (P＜0.05). Relative OD of NSBP1 in bladder cancer cell T24 treated by 0%, 10%, 40% and 80% IC50 gemcitabine were 0.492±0.044, 0.262±0.031, 0.151±0.014 and 0.089±0.011, respectively. Western blotting showed that relative OD of NSBP1 in bladder cancer cell T24 treated by 0%, 10%,40% and 80% IC50 paclitaxel were 0.473±0.017, 0.252±0.041, 0.194±0.023 and 0.118±0.016, respectively. Relative OD of NSBP1 in bladder cancer cell T24 treated by 0, 10%, 40% and 80% IC50 gemcitabine were 0.581±0.014, 0.201±0.033, 0.135±0.021 and 0.114±0.011, respectively(P＜0.05). Conclusion Paclitaxel and gemcitabine can decrease the expression level of NSBP1 in bladder cancer cell line T24 both in mRNA and protein levels, so NSBP1 may be one of the targets of chemotherapy.
