The significance of methylation status of secreted frizzled-related protein gene promoter in acute leukemia
10.3760/cma.j.issn.0578-1426.2010.09.012
- VernacularTitle:分泌型卷曲相关蛋白基因启动子甲基化状态检测在急性白血病中的意义
- Author:
Chengbo XU
;
Jianzhen SHEN
;
Songfei SHEN
;
Haiying FU
;
Yifang ZHU
;
Lu CHEN
- Publication Type:Journal Article
- Keywords:
Leukemia;
Methylation;
Secreted frizzled-related protein genes
- From:
Chinese Journal of Internal Medicine
2010;49(9):769-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between promoter hypermethylation of secreted frizzled-related protein (SFRP) gene and acute leukemia (AL). Methods We examined the promoter methylation starus of SFRP1, 2, 4 and 5 in primary or relapsed AL patients, cell lines ( HL60,NB4, Molt-4 and Jurkat) and peripheral blood mononuclear cells from healthy people with methylationspecific PCR (MSP). Results None of the normal mononuclear cells showed methylation of any SFRP genes. The frequencies of aberrant methylation among the samples were 33.9% (20/59) for SFRP1,23.7%(14/59) for SFRP2, 6. 8% (4/59) for SFRP4 and 10. 2% (6/59) for SFRP5 in acute myelocytic leukemia (AML), and 39.3% (11/28) for SFRP1, 28.6% (8/28) for SFRP2, 25.0% (7/28) for SFRP4 and 32. 1% (9/28) for SFRP5 in acute lymphoblastic leukemia (ALL). Hypermethylation of SFRP1, 2, 5genes were present in the 4 AL cell lines. SFRP4 was methylated in NB4, Molt-4 and Jurkat cell lines.However, methylation and unmethylation of SFRP4 were both detected in HL60. Conclusions Hypermethylation of SFRP genes is a common event in the evolution of AL. Methylation of SFRP genes might serve as potential independent biomarkers for early detection of AL.
