The effect of mannose-6-phosphate on adipocyte differentiation and its underlying molecular mechanism
10.3760/cma.j.issn.0578-1426.2010.09.016
- VernacularTitle:甘露糖-6-磷酸对脂肪细胞分化的影响及其分子机制初探
- Author:
Junfeng HAN
;
Yanyun GU
;
Guo LI
;
Weiping JIA
;
Min LUO
- Publication Type:Journal Article
- Keywords:
Cathepsins;
Adipocytes;
Cell differentiation;
Mannose-6-phosphate
- From:
Chinese Journal of Internal Medicine
2010;49(9):785-788
- CountryChina
- Language:Chinese
-
Abstract:
Objective Cathepsin K (CTSK) played an important role in adipocyte differentiation.The activation of CTSK needs to convey by mannose-6-phosphate receptors (M6PR) in osteoclasts. The aim of the present study was to identify the effects of mannose-6-phosphate (M6P) in adipocyte differentiation and its underlying molecular mechanism. Methods Oil red O staining, accumulation of cytoplasmic triglycerides and glycerine release were used to assess its effects on adipocyte differentiation in the 3T3-L1cell line. The enzyme activity of CTSK was observed by laser confocal microscopy. The proliferation of 3T3-L1 preadipocytes was detected by MTT methods. mRNA expression of M6PR was determined by RTPCR. Results M6P could prevent adipocyte differentiation in a dose-dependent manner as evidenced by absence of triglyceride accumulation and glycerol content. Statistical significance was showed when the concentrations of M6P were 5.0 mmol/L and 8. 0 mmol/L respectively(P ＜0. 05). The mRNA expression of M6PR was detected during the whole process of adipocyte differentiation. With the increase of M6Pconcentration, enzyme activity of CTSK was inhibited in a concentration-dependent manner. MTT method showed that the absorbance at 570 nm of 3T3-L1 preadipocytes was 0. 057 ±0. 091, increased about 62. 9%at 10. 0 mmoL/L compared with the control group (P ＜ 0. 05 ). Conclusion M6P inhibits the terminal differentiation of adipocyte, which may be associated with its effect of blocking CTSK activity by competitive binding with M6PR.
