Effects of ischemic postconditioning on neuron structure plasticity and memory after global cerebral ischemia injury in rats
10.3760/cma.j.issn.1001-8050.2010.08.029
- VernacularTitle:缺血后处理对全脑缺血损伤后神经元结构可塑性及记忆的影响
- Author:
Xin LI
;
Xinyue QIN
;
Zhenwei GUO
;
Xiaohui WU
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Dendritic spines;
Memory;
Rats
- From:
Chinese Journal of Trauma
2010;26(8):757-760
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ischemic postconditioning on neuron structure plasticity and memory after global cerebral ischemia injury in rats and discuss the protection mechanism from aspect of Morphology. Methods A total of 36 SD male rats were randomly divided into sham operation group, global cerebral ischemia for 15 min group and global cerebral ischemia plus postconditioning group, 12 rats per group. The pullsinelli 4 vessel occlusion was applied to produce the models of global cerebral ischemia reperfusion injury, common carotid arteries (CCA) occlusion with 15 min and postconditioning with three cycles, of 15 sec release and 15 sec occlusion (15s/15s). Six rats from each group were evaluated by Morris Maze test for the ability of space learning and memory and the other six rats were evaluated by golgi stain for morphologic change of neuron. Results The ischemic postcondtioning group showed significant shorter mean escape latency compared with the sham operated group ( at day 3, P =0. 014; at day 4, P =0.040; at day 5, P =0.001 ). The density of dendritic spine in ischemic postcondtioning group was increased more significantly compared with ischemic group ( F = 562. 820,P ＜ 0. 01 ). Conclusion Ischemic postconditioning has obvious protective effect on cerebral ischemiainduced memory impairment, which may be related to alleviation of dendritic spine injury.
