Effect of all-trans retinoic acid on the expression of matrix metalloproteinases-9 in perihematomal brain tissue after intracerebral hemorrhage in rats
10.3760/cma.j.issn.1673-4165.2010.07.004
- VernacularTitle:全反式维甲酸对脑出血大鼠血肿周围脑组织基质金属蛋白酶-9表达的影响
- Author:
Guangyu SHEN
;
Jie JI
;
Kefu CAI
;
Su LIU
- Publication Type:Journal Article
- Keywords:
Cerebral hemorrhage;
Matrix metalloproteinase 9;
Tretinoin;
Rats
- From:
International Journal of Cerebrovascular Diseases
2010;18(7):499-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of all-trans retinoic acid (ATRA) on the expression of matrix metalloproteinases-9 (MMP-9) in perihematomal brain tissue after intracerebral hemorrhage in rats.Methods Forty Sprague-Dawley rats were randomly allocated into ATRA and normal saline control groups.Each group was redivided into 2 h,48 h,72 h,and 7 d subgroups (n = 5 in each subgroup).The autologous blood was injected into the rat caudate nucleus for establishing a model of intracerebral hemorrhage under the guidance of stereotaxic apparatus.Intraperitoneal ATRA (1 mg/d) and the same volume of saline were injected respectively after the success of modeling.The expression of MMP-9 at different time points was detected by using immunohistochemical staining.Results The expression of MMP-9 in microvascular endothelial cells in perihematomal brain tissue in rats was upregulated 24 h after intracerebral hemorrhage in the ATRA and normal saline control groups,and it reached the peak at 48 to 72 h.The expression of MMP-9 in the ATRA group at different time points was lower than that in the normal saline control group (all P＜0.05).Conclusions ATRA inhibits the expression of MMP-9 in perihematomal brain tissue after intracerebral hemorrhage in rats,and thus may reduce the brain edema.
