The effect of nucleocapsid protein of severe acute respiratory syndrome-associated coronavirus on the expression of cyclooxygenase-2
10.3760/cma.j.issn.1000-6680.2010.08.003
- VernacularTitle:严重急性呼吸综合征冠状病毒核衣壳蛋白对环氧合酶-2表达的影响
- Author:
Chengliang ZHU
;
Yan LI
;
Xiaohong YAN
;
Yongqing TONG
;
Ming WANG
;
Zunen XIA
;
Fang LIU
- Publication Type:Journal Article
- Keywords:
SARS virus;
Nucleocapsid protein;
Cyclooxygenase 2;
Gene expression
- From:
Chinese Journal of Infectious Diseases
2010;28(8):461-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) on the expression of cyclooxygenase-2 (COX-2). Methods 293T cells were co-transfected with reporter plasmid pCOX-2-Luc containing the luciferase gene under the control of COX-2 promoter and plasmids carrying individual genes of SARS-CoV, and luciferase activity was measured. Expression of COX-2 mRNA and protein was measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results N protein of SARS-CoV enhanced COX-2 gene promoter activity, and upregulated COX-2 mRNA expression.COX-2 protein production in 293T cells was N protein concentration-dependent. Conclusion N protein of SARS-CoV could specifically activate COX-2 expression.
