Experimental studies on hematopoiesis reconstitution by using of transplantation of gene-modified bone marrow cells
- VernacularTitle:基因修饰骨髓细胞移植后重建造血的实验观察
- Author:
Ping ZOU
;
Huazhong LU
- Publication Type:Journal Article
- Keywords:
Bone marrow cell;
Gene transfer;
Transplantation
- From:
Chinese Journal of Organ Transplantation
1999;20(4):212-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological features of hematopoiesis reconstitution by using genetic marking.Methods NeoR gene was transduced into bone marrow(BM)cells of mouse mediated by liposome.Then these cells were transplanted into lethally irradiated recipients.The hematopoiesis reconstitution was observed and the marker gene in spleen and BM cells of recipients after hematopoiesis reconstitution was examined.Results The transplanted recipients remained alive and healthy.But the irradiated mice with no transplantation died from BM aplasia soon.Meanwhile,the cells from spleen and BM of transplanted mice could be alive in G418 system,and contained the DNA fragments of NeoR gene by PCR.Conclusion Gene-modified BM cells could be used to reconstitute hematopoiesis successfully and express the foreign gene to some extent stably.
