Radiosensitizing effect of gemcitabine on human lung cancer cells : A preliminary in vitro study

VernacularTitle:吉西他滨联合X射线照射对肺癌细胞系放射增敏作用的初步研究
Author: Chunling GAO ; Yuqiang CHEN ; Yunjie DAI ; Weifang SONG ; Liqing XIE
Publication Type:Journal Article
Keywords: Cell line,lung neoplasms; Gemcitabine; X-ray irradiation; Clonogenic assay; Flow cytometry; Radiosensitivity
From: Chinese Journal of Radiation Oncology 2010;19(5):471-474
CountryChina
Language:Chinese
Abstract: Objective To investigate whether gemcitabine (GEM) could enhance radiosensitivity of human non-small cell lung cancer cells and its related mechanism.Methods Clonogenic assay was used to analyze radiosensitivity enhancement by GEM on p53 mutant human lung adenocarcinoma cell line 973.Alterations of cell cycle distribution and apoptosis were measured by flow cytometry.Results Mild radiosesitizing effect was observed when 10 nmol/L GEM was administrated before or after irradiation.Marked radiosesitizing effect was demonstrated when 100 nmol/L GEM was administrated before or after irradiation, with much stronger effect of pre-irradiation GEM treatment.Mutation of p53 gene affected cell cycle redistribution and cell apoptosis, but had no relationship with radiosensitivity enhancement of GEM.Conclusions 100 nmol/L GEM could significantly enhance radiosensitivity of human lung cancer cells.However, this effect may not be associated with p53 gene mutation, cell cycle redistribution or cell apoptosis.