Protective effect of deferroxamine on glutamate induced neurotoxicity in cultured rat hippocampal neurons
10.3760/cma.j.issn.1006-7876.2010.09.015
- VernacularTitle:去铁敏对体外培养的大鼠海马神经元谷氨酸毒性的保护作用
- Author:
Yunxia LI
;
Suju DING
;
Qiang GUAN
;
Qing ZHAN
;
Zhiyu NIE
;
Lin XIAO
;
Wei GUO
- Publication Type:Journal Article
- Keywords:
Deferroxamine;
Neuroprotective agents;
Glutamic acid;
Hippocampus;
Cells cultured;
Calcium
- From:
Chinese Journal of Neurology
2010;43(9):655-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protectve effects and underlying mechanisms of deferroxamine on glutamate-induced injury in cultured hippocampal neurons.Methods Primarily cultured hippocampal neurons from fetal rat were used in a model of glutamate induced neurotoxicity.There were two experimental groups.Neurons were pretreated with deferroxamine before glutamate in the deferroxamine group, and neurons were treated with glutamate only in the control group.The morphological change was examined under microscope.Hoechst 33342 DNA staining method was used to study the ratio of condensed nuclei.The levels of lactate dehydrogenase (LDH), malonaldehyde (MDA) and hydroxyl radical were determined using biochemistry.The change in calcium signal was detected using microfluorescent technique.Results The neurons pretreated by deferroxamine had intact morphology with the ratio of condensed nuclei at 14% ± 6% compared to 58% ± 6% (t= 8.98, P ＜0.01 ) in the control group.LDH level was (36.42 ± 8.99) U/L in the deferroxamine group and was (68.06 ± 11.26) U/L in the control group ( t =3.25,P＜0.05).The respective levels of hydroxyl radical were (34.21 ±4.23) U/L and (47.06 ±8.79) U/L (t = 3.11, P ＜0.05 ).The respective levels of MDA were (12.26 ± 2.78 ) nmol/mg and (28.86±5.19) nmol/mg(t =4.88,P＜0.01).Conclusion Deferroxamine can protect neurons from glutamate induced damage.The mechanisms include an inhibition of Ca2+ overload and reduction in the levels of MDA and hydroxyl radicals.
