The mechanism of the effect of preptin on proliferation and differentiation of human osteoblasts
10.3760/cma.j.issn.1000-6699.2010.09.014
- VernacularTitle:Preptin对人成骨细胞增殖和分化的影响及机制研究
- Author:
Jiahua ZHU
;
Youshuo LIU
;
Lingqing YUAN
;
Junkun ZHAN
;
Huawen WANG
;
Eryuan LIAO
- Publication Type:Journal Article
- Keywords:
Preptin;
Human osteoblasts;
Extracellular signal regulated kinase;
Cell proliferation
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(9):784-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of preptin on proliferation and differentiation of human osteoblasts. Methods After human osteoblasts were incubated with 10-10, 10-9, 10-8 , 10-7 mol/L preptin for 24 h,the proliferation of osteoblasts was determined by[3H]thymidine incorporation and alkaline phosphatase (ALP)activity was assayed by spectrophotometric measurement. The phosphorylation levels of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase ( MAPK), extracellular signal-regulated kinase (ERK) 1/2 were assayed by Western blot. ERK inhibitor PD98059, p38MAPK inhibitor SB203580, and JNK inhibitor SP600125were used for investigating the signal pathway of preptin-stimulated osteoblast proliferation and differentiation.Results Preptin dose-dependently increased human proliferation of osteoblasts and ALP activity with the maximum effect at the concentration of l0-9 mol/L (both P<0.01 ). Preptin stimulated ERK phosphorylation in human osteoblasts, but not p38 MAPK and JNK phosphorylation. PD98059 blocked preptin-sitmulated human osteoblasts proliferation and ALP activity (both P<0.05 ), while SB203580 and SP600125 had no effect. Conclusions Preptin promotes the proliferation and differentiation of human osteoblasts through ERK pathway.
