Expression of chemokine receptors CXCR3 mRNA in peripheral blood mononuclear cells of the patients with rheumatoid arthritis by real-time fluorescence quantitative polymerase chain reaction
10.3760/cma.j.issn.1007-7480.2010.09.011
- VernacularTitle:实时荧光定量聚合酶链反应检测外周血趋化因子受体3 mRNA对类风湿关节炎的临床意义
- Author:
Guofeng WANG
;
Lianyi SHI
;
Mengxue YU
;
Liying SHEN
;
Wei GOU
;
Lihuan GUO
;
Fang XUE
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Receptor,chemokine;
Polymerase chain reaction
- From:
Chinese Journal of Rheumatology
2010;14(9):627-630
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of chemokine receptors CXCR3 mRNA in the peripheral blood mononuclear cells (PBNCs) of patients with Rheumatoid Arthritis (RA) and to analyze the relationship between the expression and the disease activity. Methods mRNA was extracted from PBNCs and the expression of CXCR3 mRNA was detected by real-time fluorescence quantitative PCR (RFQ-PCR) in 51 RA patients and 32 controls. T-test, x2-test, ANOVA were used for statistial analysis. Results Comparison between the two groups had shown that the expression levels of CXCR3 mRNA in clinical active RA group were higher than those of the RA patients in remission and healthy controls (P<0.05). The expression levels of CXCR3 mRNA were positively correlated with serum levels of ESR and CRP in clinical active RA group (r=0.824, r=0.765, P<0.05). In addition, RF titer, APF, AKA, and anti-CCP had no significant correlation with the expression levels of CXCR3 mRNA in RA patients (P>0.05). Conclusion RFQ-PCR is a sensitive,reproducible and practical test. The mRNA expressions of CXCR3 are significantly elevated in RA patients,which suggest that CXCR3 may be involved in the pathogenesis of RA. The mRNA expressions of CXCR3 in active RA patients are higher than those of RA patients in remission. These results indicate that CXCR3 may play an important role in the pathogenesis and progression of RA, and CXCR3 may be considered as an indicator for disease activity, therapeutic efficacy and prognosis of RA.
