Finding out the internal standard in place of desispramine to quantify imipramine hydrochloride by HPLC method
- Author:
Trinh Van Lau
- Publication Type:Journal Article
- Keywords:
Imipramin hydroclorid, HPLC
- MeSH:
Imipramine;
Chromatography;
High Pressure Liquid
- From:Pharmaceutical Journal
2005;0(12):20-24
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Amitriptylin hydrochloride is used as the internal standard in place of desipramine hydrochloride to determine the amount of imipramine hydrochloride by HPLC method. Results: Amitriptylin have the same structure as imipramin and the resolution degree between amitriptylin and imipramin is 1.8 while resolution degree between iminodibenzyl and imipramin is very high (R=10.7) because molecule structure of iminodibenzyl do not have branch-circuit. The suitability of chromatographic system through coefficient got high stable results with RSD<1.0%. Amitryptilin hydrochloride can be used as the internal standard in place of desipramine hydrochloride if necessary to test splitting degree in determination of imipramine hydrochloride
