Effects of different immunosuppressive agents on mesangial cell proliferation
10.3760/cma.j.issn.0254-1785.2010.09.010
- VernacularTitle:不同免疫抑制剂对肾小球系膜细胞增殖的影响
- Author:
Guobiao LIANG
;
Guangheng LUO
;
Jun SONG
;
Li YANG
;
Liyuan ZHANG
;
Shunwen LUO
;
Xianding WANG
;
Zhiyuan XIE
;
Ke WU
;
Youping LI
;
Yiping LU
- Publication Type:Journal Article
- Keywords:
Cyclosporin;
Tacrolimus;
Sirolimus;
Mycophenolatemofetil;
HBZY- 1 cell;
In vitro
- From:
Chinese Journal of Organ Transplantation
2010;31(9):545-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different immunosuppressive agents on mesangial cell proliferation through a mesangial cell injury model in vitro. Methods Mesangial cell line (HBZY-1) in period of proliferation was cultured in vitro with cytochalasin B for 2 h, then HBZY-1 cells were divided into 5 groups: blank (control) group, cyclosporine A (CsA) group, Tacrolimus (Tac) group, mycophelonate mofetil (MMF) group and rapamycin (RAPA) group. Subsequently,the number of HBZY-1 cells at different time points was measured by using the professional image analysis software after treatment for 6, 12 and 24 h, respectively. Results Damaged HBZY-1 cells recovered in all groups. At 6 h, the number of HBZY-1 cells in Tac group was significantly more than that in control group (P＜0.05), but the difference had no significance between the other treatment groups and control group (P＞0. 05). At 12 h, there was no significant difference in of the number of HBZY-1 cells among the all groups (P＞0. 05). At 24 h, there was no significant difference in the cell number between MMF and control groups (P＞0. 05). CsA, Tac and RAPA resulted in HBZY-1 cell proliferation, and the cell number in CsA and Tac groups was significantly more than that in the other groups (P＜0. 05). As compared with the control group, the cell number in RAPA group was significantly increased (P＜0. 05). Conclusion CsA, Tac, MMF and RAPA contribute to recovery of damaged HBZY-1 cells, but CsA and Tac result in over-proliferation of HBZY-1 cells. RAPA and MMF can prevent HBZY-1 cells against over-proliferation, and MMF scarcely results in HBZY-1 cell proliferation.
