Effect of 5-azacytidine on expressions of atrial natriuretic peptide and β-myosin heavy chain in cultured rat bone marrow stromal cells
10.3760/cma.j.issn.0254-9026.2010.09.017
- VernacularTitle:5-氮胞苷对大鼠骨髓基质细胞心钠素及β-肌球蛋白重链表达的影响
- Author:
Yang ZHAO
;
Xinzheng LU
- Publication Type:Journal Article
- Keywords:
Azacytidine;
Atrial natriuretic factor;
Myosin heavy chain
- From:
Chinese Journal of Geriatrics
2010;29(9):761-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of 5-azacytidine (5-AZ) on the expressions of atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC) genes in cultured rat bone marrow stromal cells (BMSCs). Methods Neonatal rat cardiomyocytes and adult rat BMSCs were isolated and cultured. BMSCs were incubated with 5-AZ and (or) myocardiocytes culture medium. RT-PCR was used to measure the expressions of cardiac ANP and β-MHC genes. Results Normally cultured rat BMSCs didn't express cardiac ANP or β-MHC. After inducing by 5-AZ, rat BMSCs expressed ANP or β-MHC. The myocardiocytes culture medium enhanced expression of β-MHC, but didn't affect the expression of ANP. Conclusions The 5-AZ induces the rat BMSCs to express either cardiac ANP or β-MHC, and the former could be enhanced by cardiomyocytes culture medium.
