Resveratrol inhibits matrix metalloproteinases-9 expression induced by soluble CD40 ligand in macrophages
10.3760/cma.j.issn.0254-9026.2010.09.018
- VernacularTitle:白藜芦醇抑制可溶性CD40配体作用下巨噬细胞基质金属蛋白酶-9的表达
- Author:
Pengli ZHU
;
Dean JIA
;
Yanghui SHEN
;
Jingming RUAN
;
Huizhen YU
;
Hui CHEN
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Macrophages;
Matrix metalloproteinases
- From:
Chinese Journal of Geriatrics
2010;29(9):764-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of resveratrol on the expression of matrix metalloproteinases-9 (MMP-9) in soluble CD40 ligand (sCD40L)-activated macrophages. Methods Human monocytic cell line THP-1 cells under an inducing of phorbol ester differentiated into macrophages. Then the macrophages were sitimulated by sCD40L independently and after a preincubation with resveratrol. The mRNA expression of MMP-9 and tissue-inhibitor of metalloproteinase-1 (TIMP-1) in macrophages were investigated by semiquantitative RT-PCR. The secretions of MMP-9 and TIMP-1 protein were measured by Western blot. The MMP-9 activity was analyzed by gelatin zymography technique. Results The expressions of MMP-9 gene(1.53±0.04 vs. 0.75±0.01,P＜0.05) and protein(244 930.8±31 268.6 vs. 192 976.8±20 223.1,P＜0.05)were higher in macrophages when stimulated by sCD40L. Resveratrol (10 μmol/L and 50 μmol/L)can inhibit the CD40L-induced gene expression and the protein secretion of MMP-9 (P＜0.01). The activity of MMP-9 was degraded by resveratrol (P＜0.05). Meanwhile resveratrol could increase the gene expression and protein secretion of TIMP-1 (P＜0.05). Conclusions Resveratrol can inhibit the CD40L-activated macrophage expression of MMP-9. It may be one of its mechanisms on antiatherosclerosis and stabilization of atheromatous plaques.
