Oral Administration of Glycine and Leucine Dipeptides Improves Skin Hydration and Elasticity in UVB-Irradiated Hairless Mice.
10.4062/biomolther.2017.089
- Author:
Jong Il PARK
1
;
Ji Eun LEE
;
Hyun Jun SHIN
;
Soonran SONG
;
Won Kyung LEE
;
Jae Sung HWANG
Author Information
1. Department of Genetic Engineering & Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea. jshwang@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Gly-Leu;
Leu-Gly;
Hyaluronan;
elasticity;
Porcine placenta extract
- MeSH:
Administration, Oral*;
Animals;
Dipeptides*;
Elasticity*;
Glycine*;
Humans;
Hyaluronic Acid;
In Vitro Techniques;
Intercellular Signaling Peptides and Proteins;
Keratinocytes;
Leucine*;
Mice;
Mice, Hairless*;
Minerals;
Miners;
Pancreatic Elastase;
Peptides;
Placenta;
RNA, Messenger;
Skin*;
Up-Regulation
- From:Biomolecules & Therapeutics
2017;25(5):528-534
- CountryRepublic of Korea
- Language:English
-
Abstract:
Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leucyl-L-Leucine (Leu-Leu), affect skin hydration and elasticity in vitro and in vivo. We found that Gly-Leu and Leu-Gly dipeptides induced the expression of transglutaminase 1 in normal human epidermal keratinocytes (NHEKs) whereas Leu-Leu dipeptides did not. Treatment with Gly-Leu or Leu-Gly significantly increased hyaluronan (HA) synthesis in NHEKs and the upregulation of hyaluronan synthase 2 (HAS2) mRNA level was confirmed. In addition, elastase activity was inhibited in NHEKs treated with Gly-Leu or Leu-Gly dipeptides. Oral administration of Gly-Leu or Leu-Gly dipeptides increased skin hydration and elasticity in UVB-irradiated hairless mice. The significant upregulation of HA in UVB-irradiated hairless mice was observed in response to oral administration of Gly-Leu or Leu-Gly. These results suggest that the major dipeptides of porcine placenta, Gly-Leu and Leu-Gly, are potentially active ingredients for skin moisturization formulations.
