Effects of different concentrations of isofluraue on viability in rat primary cortical neurons
10.3760/cma.j.issn.0254-1416.2010.06.012
- VernacularTitle:不同浓度异氟醚对新生大鼠离体大脑皮层神经元活力的影响
- Author:
Qiujun WANG
;
Xiuli WANG
;
Juan ZHAO
;
Zhujun ZHAO
;
Yanxia Lü
;
Hongxia ZHU
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Neurons;
Cerebral cortex;
Cell survival
- From:
Chinese Journal of Anesthesiology
2010;30(6):673-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of isoflurane on viability in rat primary cortical neurons. Methods Primary cortical neurons were isolated from neonatal Wistar rats (less than 24 h after birth) and exposed to 0.6 %, 1.2 % and 2.4 % isoflurane for 1, 2, 4, 8, 12 or 24 h, cell viability was evaluated by MTT reduction and LDH release assays. Changes in intracellular [Ca2+]i were detected by real-time confocal microscopy after the neurons were exposed to different concentrations of isoflurane. Results Exposure to 0.6% isoflurane for 24 h significantly increased viability of the primary rat cortical neurons as shown by decrease in LDH release and increase in MTT and elevation of peak [Ca2+]i. Exposure to 1.2% isoflurane for 12 or 24 h and to 2.4% isoflurane for 8, 12 or 24 h significantly reduced viability of cortical neurons associated with high and fast elevation of peak [Ca2 +] i. Conclusion Exposure to 0.6 % isoflurane increases viability of the primary rat cortical neurons. Exposure to 1.2% isoflurane and to 2.4% isoflurane decreases cell viability and the mechanism may be related to the changes in calcium concentrations in the neurons.
