Inhibitory Effect of Carnosol on Phthalic Anhydride-Induced Atopic Dermatitis via Inhibition of STAT3.
10.4062/biomolther.2017.006
- Author:
Do Yeon LEE
1
;
Chul Ju HWANG
;
Ji Yeon CHOI
;
Mi Hee PARK
;
Min Ji SONG
;
Ki Wan OH
;
Dong Ju SON
;
Seung Hwa LEE
;
Sang Bae HAN
;
Jin Tae HONG
Author Information
1. College of Pharmacy and Medical Research Center, Republic of Korea. jinthong@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Atopic dermatitis;
STAT3;
Carnosol
- MeSH:
Animals;
Dermatitis, Atopic*;
DNA;
Inflammation;
Mice;
Nitric Oxide;
Phenol;
Phosphorylation;
RAW 264.7 Cells;
Serum;
Skin
- From:Biomolecules & Therapeutics
2017;25(5):535-544
- CountryRepublic of Korea
- Language:English
-
Abstract:
Carnosol is a phenolic antioxidant present in rosemary (Rosmarinus officinalis). It is known for anti-inflammatory effects, analgesic activity and anti-cancer effects. However, no study has been dedicated yet to its effect on atopic dermatitis (AD). Here, we show that carnosol effectively inhibited LPS-induced nitric oxide (NO) generation and expression of inflammatory marker proteins (iNOS and COX-2) in RAW 264.7 cells. In addition, carnosol effectively inhibits the phosphorylation of STAT3 and DNA binding activity in RAW 264.7 cells. Pull down assay and docking model analysis showed that carnosol directly binds to the DNA binding domain (DBD) of STAT3. We next examined the anti-atopic activity of carnosol (0.05 μg/cm²) using 5% Phthalic anhydride (PA)-induced AD model in HR1 mice. Carnosol treatment significantly reduced 5% PA-induced AD like skin inflammation in skin tissues compared with control mice. Moreover, carnosol treatment inhibits the expression of iNOS and COX-2 in skin tissue. In addition, the levels of TNF-α, IL-1β, and Immunoglobulin-E in blood serum was significantly decreased in carnosol treated mice compared with those of 5% PA treated group. Furthermore, the activation of STAT3 in skin tissue was decreased in carnosol treated mice compared with control mice. In conclusion, these findings suggest that carnosol exhibited a potential anti-AD activity by inhibiting pro-inflammatory mediators through suppression of STAT3 activation via direct binding to DBD of STAT3.
