In vitro susceptibilities of urogenital Chlamydia trachomatis to five antimicrobial agents alone or in combination
10.3760/cma.j.issn.0412-4030.2010.10.005
- VernacularTitle:五种常用抗菌药物对泌尿生殖道沙眼衣原体的单独和联合药敏作用
- Author:
Mei WANG
;
Yong JIANG
;
Lili SHAO
;
Yuanjun LIU
;
Shuping HOU
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Anti-infective agents,urinary;
Microbial sensitivity tests;
Drug interactions
- From:
Chinese Journal of Dermatology
2010;43(10):687-690
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the in vitro acitivity of azithromycin, minocyline, moxifloxacin, doxycycline and rifampicin alone or in combination against three standard strains of urogenital Chlamydia trachomatis,i.e. D-UW-5/Cx, E-UW-5/Cx and G-UW-5/Cx. Methods Three standard strains of C. trachomatis were inoculated into McCoy cells, and used to susceptibility testing after more than 90% McCoy cells were infected.Microdilution method was applied to determine the activity of the 5 antimicrobial agents alone, and checkerboard array to determine that in combination. Results The in vitro minimal inhibitory concentrations (MICs)were 0.25 mg/L for azithromycin, 0.06 mg/L for moxifloxacin, 0.063 - 0.25 mg/L for doxycycline, 0.032 -0.064 mg/L for minocyline, 0.008 mg/L for rifampicin. And, the fractional inhibitory concentration index was constantly 0.75 for the combination of azithromycin with moxifloxacin, doxycycline and rifampicin, 2.5, 2.83and 4 for the combination of minocyline with azithromycin, moxilloxacin and rifampicin, respectively. Conclusions As far as the activity against C. trachomatis is concerned, there is a synergism for the combination of azithromycin with moxifloxacin, doxycycline and rifampicin, but antagonism for the combination of minocyline with azithromycin, moxifloxacin and rifampicin.
