Analysis of haemagglutinin gene of the first influenza A-H1N1 viral strain isolated in Guangdong Province during an influenza A pandemic in 2009
10.3760/cma.j.issn.1000-6680.2010.09.003
- VernacularTitle:2009年广东省甲型流行性感冒暴发流行期间首株甲型H1N1流行性感冒病毒血凝素基因分析
- Author:
Shaojing WEI
;
Chunhua XIAO
;
Weilie CHEN
;
Jian WANG
;
Jie LUO
;
Fuchun ZHANG
;
Chibiao YIN
;
Zhan YANG
;
Weidong JIA
- Publication Type:Journal Article
- Keywords:
Orthomyxoviridae infections;
Influenza A virus,H1N1 subtype;
Protein isoforms;
Genes,viral;
Reverse transcriptase polymerase chain reaction;
Nucleic acids;
Sequence analysis
- From:
Chinese Journal of Infectious Diseases
2010;28(9):524-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect and analyze the haemagglutinin (HA) gene of the first influenza A-H1N1 viral strain isolated in Guangdong Province during an influenza A pandemic in 2009.Methods A-H1N1 virus strain was isolated from the throat swab of the first patient diagnosed with A-H1N1 virus infection in Guangdong Province in 2009. Viral nucleonic acid was extracted from supernatant of cell culture and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR) with HA gene-specific primers. The product was cloned, sequenced, and the homology was analyzed. Results A 1710 bp HA gene of the first influenza A-H1N1 viral strain in Guangdong Province in 2009 was acquired, which was named as A/GuangzhouSB/01/2009 (H1N1) HA with GenBank access No. GQ268003. The homology of the studied HA gene and the 277 influenza A (H1N1) isolates reported in the epidemic areas was 99.0%-99.8%, and as high as 99.8% when compared with the isolates reported in the United States where the patient had traveled. When the studied HA gene was compared with 25 isolates of Chinese seasonal A-H1N1 virus, the homology was 72.3%-85.6%. Conclusions The homology of the first isolated A-H1N1 viral strain in Guangdong Province in 2009 and epidemic influenza A-H1N1 virus is high, while it is low compared with Chinese seasonal A-H1N1 virus.
