Dynamic analysis of CD127 expression on the memory CD8+ lymphocytes of hepatitis B e antigen positive chronic hepatitis B patients treated with peginterferon α-2a
10.3760/cma.j.issn.1000-6680.2010.09.007
- VernacularTitle:聚乙二醇干扰素α-2a对乙型肝炎病毒e抗原阳性慢性乙型肝炎患者记忆性CD8+T淋巴细胞CD127表达影响的动态研究
- Author:
Lingjun YING
;
Lin ZHENG
;
Guocai Lü
;
Chenhuai XU
;
Wei WU
;
Yida YANG
- Publication Type:Journal Article
- Keywords:
CD8-positive T lymphocytes;
Interleukin-7 receptor alpha subunit;
Polyethylene glycols;
Interferon alfa-2a;
Hepatitis B,chronic;
Hepatitis B e antigens;
Gene expression
- From:
Chinese Journal of Infectious Diseases
2010;28(9):541-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze CD127 expression on the memory CD8+ lymphocytes from hepatitis B e antigen (HBeAg) positive chronic hepatitis B (CHB) patients treated with peginterferon α-2a (Pegasys). Methods Thirty HBeAg positive CHB patients were treated with peginterferon α-2a 180 μg once a week for 48 weeks and followed up for 24 weeks. The memory CD8+ lymphocytes were characterized by expressing CD45RA and CD27 markers. CD127 expression on cell surface was measured by four-colour flow cytometry. The difference of mean values between groups was evaluated by Mann-Whitney test. Results The CD127 expression on CD8+ T lymphocytes was significantly lower in HBeAg positive CHB patients compared to healthy controls (Z=2.889, P＜0.05), which was negatively correlated with serum hepatitis B virus (HBV) DNA level and HBeAg titers. The CD127 expression increased along with the decrease of HBV DNA and HBeAg after 24-week, 48-week and 72-week treatment in patients showing good response to peginterferon α-2a, while CD127 expression didn't change markedly in non responders (Z24w = 1.954, Z48w = 2.789, Z72w = 2. 989; all P＜0. 05). Conclusion CD127 expression on memory CD8+ lymphocytes increases along with effective anti-HBV treatment in CHB patients, which can be used as a marker for evaluating the effectiveness of anti-viral treatment.
