The interference of picroside Ⅱ on the expressions of Caspase-3 following cerebral ischemia reperfusion injury in rats
10.3760/cma.j.issn.1673-4246.2010.05.001
- VernacularTitle:胡黄连苷Ⅱ对大鼠脑缺血再灌注损伤Caspas-3表达的影响
- Author:
Lei FANG
;
Li SUN
;
Xiangcong GONG
;
Huanmin GAO
- Publication Type:Journal Article
- Keywords:
Picroside Ⅱ;
Cerebral ischemia;
Reperfusion injury;
Caspase-3;
Apoptosis
- From:
International Journal of Traditional Chinese Medicine
2010;32(5):389-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of picrodideⅡ on the expressions of Caspase-3 in brain tissue following cerebral ischemic reperfusion injury in rats. Methods The middle cerebral artery occlusion reperfusion models were established with intraluminal thread methods in rats. Picrodide Ⅱ (10 mg/kg) was injected from tail vein for treatment. The neurological function was evaluated with Bederson's test. The brain structure was observed by hematoxylin-eosin (HE)stain and the apoptosis were counted by TUNEL immunofiuorescence assay. The expressions of Caspase-3 were detected with immunohistochemical and enzyme linked immunosorbent assay. Results After ischemia for 2 h and reperfusion for 22 h, the rats showed neurological function deficit. The expressions of Caspase-3 and the number of apoptotic cells in brain tissue increased significantly than those in the sham operative group (P＜ 0.01). In picroside, the Bederson's scores, the expressions of Caspase-3 and the number of apoptosis cells were significantly lower than those in the negative control group(P＜0.01).Conclusion Picroside Ⅱ might reduce the expressions of Caspase-3 to inhibit the neuronal apoptosis induced by cerebral ischemia reperfusion injury and improve the neurological function of rats.
