Effects of ketamine combined with moderate hypothermia on brain ischemia-reperfusion injury in a rat model of asphyxial cardiac arrest
10.3760/cma.j.issn.0254-1416.2010.06.032
- VernacularTitle:氯胺酮联合亚低温对窒息性心跳骤停大鼠复苏后脑缺血再灌注损伤的影响
- Author:
Xuexin FENG
;
Yongqiang ZHANG
;
Yuliang XUE
;
Yuechun LU
;
Chunlin GAO
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
Ketamine;
Hypothermia,induced;
Asphyxia;
Heart arrest;
Reperfusion injury;
Brain
- From:
Chinese Journal of Anesthesiology
2010;30(6):733-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ketamine combined with moderate hypothermia on brain ischemia-reperfusion (I/R) injury in a rat model of asphyxial cardiac arrest. Methods Fifty healthy Wistar rats of both sexes aged 4.0-4.5 months, weighing 410-510 g were randomly allocated into 5 groups (n = 10each): group Ⅰ sham operation (group S), group Ⅱ asphyxial cardiac arrest (group ACA), group Ⅲ ketamine (group K), group Ⅳ moderate hypothermia (group MH) and group Ⅴ K + MH. The animals were anesthetized with intraperitoneal (IP) phenobarbital 20 mg/100 g, tracheostomized and mechanically ventilated (RR 60 bpm,FiO2 50%), PaCO2 was maintained at 35-45 mm Hg. Cardiac arrest was induced by clamping tracheal tube until ECG activity disappeared and MAP ＜ 15 mm Hg. Resuscitated was started 5 min later. MAP ＞ 60 mm Hg and HR ＞ 250 bpm were considered to be signs of successful resuscitation. Dead animals and animals in which resuscitation time was longer than 5 min were excluded from the study. In group K ketamine 100 mg/kg was administered IP at 5 min before asphyxia. In group MH hypothermia was started as soon as asphyxia was started and body temperature was maintained at 30-35 ℃. After successful resuscitation, the animals were sacrificed. Their brains were removed for determination of brain water content and p-caspase-3 expression in hippocampus. Results Brain I/Rsignificantly increased brain water content and p-caspase-3 expression in group ACA. MH alone significantly attenuated 1/R-induced brain edema and decreased p-caspase-3 expression, while ketamine alone only significantly decreased p-caspase-3 expression but did not decrease I/R-induced brain edema. MH + K decreased p-caspase-3expression further but did not reduce brain edema further as compared with MH alone. Conclusion Ketamine combined with moderate hypothermia provides better protection against brain I/R injury.
