The apoptosis of neuron and expression of ERK in cortex induced by endotoxemia in young rats
10.3760/cma.j.issn.1673-4912.2010.05.022
- VernacularTitle:内毒素诱导幼年大鼠脑神经细胞凋亡及脑组织ERK表达的变化
- Author:
Zhe LI
;
Yajuan ZHAO
- Publication Type:Journal Article
- Keywords:
Endotoxin;
Sepsis;
Apoptosis;
Rat
- From:
Chinese Pediatric Emergency Medicine
2010;17(5):437-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ERK in apoptosis of neuron induced by endotoxemia,provide the theory basis for the early intervention. Methods Wistar rats aged 10 day were randomly divided into two groups:LPS group and NS group. Neuron apoptosis at 6、12、72 h after abdomen injection were detected by TUNEL method, the expressions of ERK were measured by immunohistochemial method.Results ( 1 ) apoptosis cell appeared at 6 hour in LPS group, but the apoptosis index showed no difference from NS group( P ＞ 0. 05 ). The apoptosis cell obvious increased at 12 hour, peaked at 72 hour( P ＜ 0. 05 ,P ＜0. 01 ). (2) As compared with NS group, the expression of ERK increased at 6 h ( P ＜ 0. 01 ), obviously decreased at 12 h(P ＜0. 01 ) ,and reversed to the level of NS group at 72 h(P ＞0. 05). Conclusion LPS can induce the neuron apoptosis in young rats, the ERK is activated and could play role in apoptosis.
