Comparison of the effect of ibuprofen and glucosamine on synoviocyte proliferation and cartilage oligomeric matrix protein expression in knee osteoarthritis of human
10.3760/cma.j.issn.1007-7480.2010.10.004
- VernacularTitle:布洛芬与氨基葡萄糖对膝骨关节炎患者滑膜细胞增殖及软骨寡聚基质蛋白表达影响的比较
- Author:
Peng ZHANG
;
Yuxin ZHENG
;
Yuelong CAO
;
Guantong SHI
- Publication Type:Journal Article
- Keywords:
Synovial membrane;
Osteoarthritis,knee;
Extracellular matrix proteins;
Cartilage oligomeric matrix protein
- From:
Chinese Journal of Rheumatology
2010;14(10):668-671
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of ibuprofen and glucosamine on synoviocyte proliferation and cartilage oligomeric matrix protein (COMP) expression in human knee osteoarthritis. Methods Human synoviocytes were isolated from synovium (earlier stage and late stage of OA) by tissue culture and were cocultured with ibuprofen and glucosamine. The concentration of COMP was determined by MTS/PMS method and hCOMP kit. Two-tailed t-test was used for statistical analysis. Results The observation time of tissue culture was determined at 5～7 day by the MTS/PMS method. The A values of glucosamine [ late stage group (0.054±0.021), early stage group (0.777±0.034)] were less than the normal serum control group (P＜0.05).Both ibuprofen [late stage group (35.4±1.9), early stage group (46.0±2.2)] and glucosamine [late stagegroup (36.6±1.3), early stage group (48.8±1.3) ] could decrease the concentration of COMP in synoviocyte secretion in vitro (P＜0.05). Conclusion Glucosamine can inhibit the synoviocyte proliferation of human knee osteoarthritis (both early stage and late stage) in vitro. Both ibuprofen and glucosamine can inhibit the COMP secretion of synoviocyte in vitro.
