Study on differentiation of adipose-derived stem cells into neural cells induced by supernatant liquid from brain tissue in rats
10.3760/cma.j.issn.0254-9026.2010.10.020
- VernacularTitle:大鼠脑组织上清液诱导脂肪来源干细胞分化为神经细胞的实验研究
- Author:
Wei WANG
;
Yunhai LIU
;
Ning ZHANG
;
Xingdong YANG
;
Qidong YANG
;
Xiangmin SHEN
;
Liang YANG
- Publication Type:Journal Article
- Keywords:
Stem cells;
Cell differentiation;
Cell,cultured;
Brain ischemia
- From:
Chinese Journal of Geriatrics
2010;29(10):849-853
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of supernatant liquid from brain tissue on the differentiation of adipose-derived stem cells (ADSCs) into neural cells in normal brain tissues, the homogenate of infarcted cerebral hemisphere and the opposite side in the rats. Methods The ADSCs were obtained from rat retroperitoneal adipose tissue. The normal brain tissues, the homogenate of the infarcted cerebral hemisphere and thc opposite side got from middle cerebral artery occlusion in rats were used to induce ADSCs. Immunocytochemistry or immunofluorescence were used to identify the cell types at the 3rd day. Positive expression rate was counted by fluorescence microscope. Results (1)The neuron-specific enolase (NSE) positive cells, microtubule-associated protein 2 (MAP-2) positive cells and glial fibrillary acidic protein (GFAP) positive cells were much more in the homogenate of the infracted cerebral hemisphere than in others (P＜0.05). (2)The NSE positive cells, MAP-2 positive cells and GFAP positive cells were much more in the homogenate of the normal brain tissues and the opposite side than normal level ( P ＜ 0. 05 ) . Conclusions The homogenate of the infracted cerebral hemisphere and the opposite side can induce adipose-derived stem cells into neural-like cells and express neural cells markers in rats.
