Correlation of quantititive measurements between HBV-DNA and HBV-M
10.3760/cma.j.issn.1008-6315.2010.10.021
- VernacularTitle:HBV-DNA和HBV-M定量检测的相关性研究
- Author:
Yueqiong CHEN
;
Wanyun ZENG
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
HBV-DNA;
HBV-marker
- From:
Clinical Medicine of China
2010;26(10):1065-1067
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between HBV-DNA and serum HBV markers quantitative detection and determine its diagnosis value in hepatitis B virus infection. Methods The HBV-DNA was measured by fluorescence quantitative PCR and hepatitis B virus was detected by time-resolved fluorescence immunoassay (TRFIA). The relationship between the two tests was analyzed statistically using software. Results Detection of HBV-DNA in the peripheral blood of patients with HBsAg (+), HBeAg (+), HBcAb ( + ) showed 92. 9% (79/85) positive rate,and the average DNA copy number was (4.31 ± 1.64) × 106 copies/ml. While the detection of HBV-DNA in the peripheral blood of patients with HBsAg ( + ), HBeAb ( + ), HBcAb ( + ) showed 47.7% (105/220) positive rate, and the average DNA copy number was (2.47 ± 2. 21 ) × 104 copies/ml. In normal controls,blood HBV-DNA detection was negative. Furthermore, HBV-DNA copy number correlated positively with HbeAg quantitation (r = 0. 59, P = 0. 041 ). However, we found no significant correlation between HBV-DNA copy number and HbsAg quantititation (r = 0.221, P = 0.077). Conclusions Different HBV-M had diverse correlation with HBV-DNA number, comprehensive detection of them would be better to assist clinical diagnosis and evaluate the response to treatments.
