Treatment of pancreatic cancer by replicating adenovirus mediated human endostain gene in nude mice
10.3760/cma.j.issn.1007-631X.2010.10.004
- VernacularTitle:增殖型腺病毒介导的人内皮抑素基因治疗胰腺癌
- Author:
Xueqiang WANG
;
Yifeng FANG
;
Heping Lü
;
Yunfeng SHAN
;
Qiyu ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Gene therapy;
Adenovirus,human;
Endostatin
- From:
Chinese Journal of General Surgery
2010;25(10):797-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of a dual regulation of replicating adenovirus vector carrying human endostatin gene (AdTPHre-hEndo) on pancreatic cancer. Methods Human endostatin (hEndo) gene was cloned into the genome of replicating adenovirus specific for the tumor cells by virus recombination technology. The virus titer was 3.25 × 1010pfu/ml. A Balb/c nude mouse model carring sw1990 cells pancreatic cancer was established, the expression of human endostain and inhibition of tumor cells in vivo were detected. Results We successfully constructed AdTPHre-hEndo. The inhibition on pancreatic cancer cell line SW-1990 of AdTPHre-hEndo is better than Ad-hEndo (P ＜0. 01 ), and ONYX-015 ( P ＜ 0. 05 ). The endostatin expression of AdTPHre-hEndo group was significantly higher than Ad-hEndo group and the control group (P ＜ 0. 01 ). The intratumoral MVD also decreased significantly in the treated tumors(6. 8 ±2. 5 vs. 16. 0 ±4. 6、47. 2 ± 10. 0, P ＜0. 01 ). Conclusions The recombination adenovirus can express biologically active hEndo effectively, which results in inhibiting the growth of micro-blood vessels and proliferation slowly.
