Human leukocyte antigen-G 14 bp deletion polymorphism in severe pre-eclampsia
10.3760/cma.j.issn.0529-567x.2010.05.007
- VernacularTitle:人类白细胞抗原G基因14 bp缺失多态性与重度子痫前期发病的关系
- Author:
Zhan ZHANG
;
Jingyan WANG
;
Linlin ZHANG
;
Shang GUO
;
Liting JIA
;
Hui LI
;
Jing LI
;
Juan JU
;
Shouhua RONG
- Publication Type:Journal Article
- Keywords:
Pre-eclampsia;
HLA antigens;
Histocompatibility antigens class Ⅰ;
Polymorphism,genetic;
Sequence deletion
- From:
Chinese Journal of Obstetrics and Gynecology
2010;45(5):348-352
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between human leukocyte antigen-G( HLA-G) gene Exon 8 14 bp deletion polymorphism and the pathogenesis of severe pre-eclampsia.Methods Forty-two pregnant women with severe pre-eclampsia,who admitted to the Third Affiliated Hospital of Zhengzhou University from October 2008 to February 2009,and their newborns were chosen as the severe pre-eclampsia group.Another 45 healthy gravidas at the third trimester and their newborns were chosen as the control.All gravidas in both groups were Han Nationality.HLA-G Exon 8 genotyping was detected by PCR in both groups and the allele frequencies and genotype frequencies were compared between the two groups.The genotype frequencies of maternal-neonatal pairs were also analyzed.Results ( 1 ) In the severe pre-eclampsia group,14% of the maternal-neonatal pairs were homozygote of 14 bp deletion,and significantly higher frequency 33% (15/45) was found in the control group (P =0.038).(2) No significant difference was found in the allele frequencies and genotype frequencies of HLA-G 14 bp deletion polymorphism among all the mothers between the two groups (P >0.05).(3) The + 14 bp and-14 bp allele frequencies of HLA-G 14 bp deletion polymorphism in newborns in the severe pre-eclampsia group were 44% (37/84) and56% (47/84),respectively,and 30% (27/90) and 70% (63/90) in the control group.Although there was no significant difference between the two groups,but differences in trends was identified (χ2= 3.678 P = 0.055) ; The genotype (-14 bp/-14 bp) frequency of neonates in the severe pre-eclampsia group showed no difference compared with that in the control group[29% (12/42) vs 49% (22/45)],but differences in trends was also found (P =0.052).Conclusions HLA-G 14 bp deletion polymorphism is associated with the susceptibility of severe pre-eclampsia in Chinese Han nationality.Maternal-fetal genotype pairs of-14 bp/-14 bp may have reduced risk of severe pre-eclampsia.
