The immunomodulatory effect of allogenic mesenchymal stem cell transplantation on rats with collagen induced arthritis
10.3760/cma.j.issn.1007-7480.2010.10.007
- VernacularTitle:异基因骨髓间充质干细胞移植对胶原诱导性关节炎大鼠的免疫调节作用
- Author:
Fang LI
;
Xiaofeng LI
;
Jieting JIA
;
Liyun ZHANG
;
Lihui MA
;
Ke XU
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Arthritis,experimental;
Mesenchymal stem cell
- From:
Chinese Journal of Rheumatology
2010;14(10):682-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the immunologic effect of transplantation of MSCs by studying the early and later period of collagen induced arthritis. Methods Rats MSCs were isolated and expanded from bone marrow cells by density gradient centrifugation and adhering to the culture plastic bottle, and the phenotypes were assessed by flow cytometry. We established collagen induced arthritis rats model. MSCs wereinjected from tail veins. We observed the expression of Foxp3 mRNA using RT-PCR, and the level of CD4+CD25+ T cell was tested by flow cytometry. One-way ANOVA and LSD-t test were used for statistical analysis.Results The percentage of CD4+ CD25+ T cells in early and later CIA groups was lower than that of normal control group and treatment groups, which showed statistically significant difference (P＜0.05). The level of CD4+ CD25+ T cell in early MSCs treatment group was higher than the later MSNs treatment group, which showed statistically significant difference (P＜0.05). Compared to the normal group and treatment groups, the expression level of Foxp3 mRNA in the early and later CIA groups was decreased markedly, while the early MSNs treatment group versus the later treatment group showed no statistically significant difference (P＞0.05).The intensity of Foxp3 mRNA in the treatment groups was similar to normal control group. Conclusion In this study, MSCs has shown significant immune-modulatory effects. It up-regulates the level of CD4+ CD25+ T cell in CIA rats, accelerate the expression of Foxp3 mRNA. The early treatment group is more effective than the late treatment group.
