Study on histone acetylation modulation and Akt signaling pathway inhibition by phenyhexyle isothiocyanate in prostate cancer PC3 cell line

VernacularTitle:异硫氰酸苯己酯对前列腺癌PC3细胞组蛋白乙酰化及Akt信号转导通路的影响
Author: Zhiming ZHUANG ; Xudong MA ; Yiqun HUANG ; Zhouda ZHENG ; Yacai ZHENG ; Shaohong JIANG
Publication Type:Journal Article
Keywords: Phenyhexyle isothiocyanate; Prostatic neoplasms; Akt signaling pathway; Histone; Acetylation
From: Chinese Journal of Urology 2010;31(10):707-709
CountryChina
Language:Chinese
Abstract: Objective To investigate phenyhexyle isothiocyanate (PHI) modulating histone acetylation and inhibiting Akt signaling pathway in prostate cancer cell line PC3 in vitro. Methods Apoptotic cells were measured by TUNEL assay. Histone acetylated H3, H4 and the Akt protein signaling pathway (Akt, p-Akt, mTOR, p-mTOR, p70S6K and p-p70S6K) were detected by Western blot. Results Apoptotic cells increased after exposure to PHI with concentration dependent. PHI significantly induced an accumulation of histone acetylated H3, H4. The change of Akt, mTOR, p70S6K proteins was not observed. Phosphorylation of Akt (p- Akt), mTOR (p-mTOR) and p70S6K (p-p70S6K) decreased after exposure to PHI for 7 h. Conclusions PHI can induce histone acetylation H3, H4 accumulation. PHI inhibits Akt signaling pathway resulting cell apoptosis. It might be a new anticancer agent.