Effects of O-GIcNAc modification on glutamine-induced heat shock protein 70 expression in LPS-treated rat cardiomyocytes
10.3760/cma.j.issn.0254-1416.2010.04.035
- VernacularTitle:O-GlcNAc修饰在谷氨酰胺诱导LPS干预的大鼠心肌细胞HSP70表达中的作用
- Author:
Junsong GONG
;
Liang JING
- Publication Type:Journal Article
- Keywords:
Protein processing,post-translational;
HSP70 heat-shock proteins;
Gintamine;
Endotoxins;
Myocytes,cardiac
- From:
Chinese Journal of Anesthesiology
2010;30(4):500-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of O-GlcNAc modification on gintamine (Glu)-induced heat shock protein 70 (HSP70) expression in LPS-treated rat cardiomyocytes.Methods Primary cultures of neonatal rat cardiomyocytes were randomly divided into 6 groups:group Ⅰ control(group C);group Ⅱ Glu;group Ⅲ LPS;group Ⅳ Glu+LPS;group Ⅴ Glu+LPS+Alloxan and group Ⅵ Gln+LPS+PUGNAc.In group C double distilled water 25 μl was added.In group Ⅱ-Ⅵ the cells were exposed to the sanle concentrations of Glu (5 mmol/L)and LPS(4 μg/ml) except Alloxan (an inhibiter of O-linked β-N-acetyl glucosamine transferase/OGT) (1 mmol/L) and PUGNAc (an inhibitor of O-linked β-N-acetyl glucosaminidase/OGA)(100μmol/L).After being incubated for 6 h,cardiomyocyte viability,O-GlcNAc modification level and HSP70 expression level were measured.Results There was no significant difference in cell viability among the six groups.The levels of O-GlcNAc modification and HSP70 expression were significantly higher in group Ⅱ-Ⅵ than in group Ⅰ,were significantly higher in group Ⅳ and group Ⅵ than in group Ⅲ,were significantly lower in group Ⅴ and higher in group Ⅵ than in group Ⅳ.Conclusion O-GlcNAc modification may be involved in Glu-induced HSPT0 expression in LPS-treated cardiomyocytes.
