Study on the senescence of rat glomerular mesangial cells induced by Tert-Butyl hydroperoxide in vitro and the influnce of probucol on its senescence
10.3760/cma.j.issn.1008-6315.2010.07.022
- VernacularTitle:叔丁基过氧化氢体外诱导鼠系膜细胞衰老及普罗布考对其影响的研究
- Author:
Hongli ZHOU
;
Yarong HAN
;
Ruixia JIN
;
Bo HUANG
- Publication Type:Journal Article
- Keywords:
Tert-Butyl hydroperoxide;
Human glomerular mesangial cells;
Cell senescence;
Probucol
- From:
Clinical Medicine of China
2010;26(7):733-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the senescence of rat mesangial cells induced by Tert-Butyl hydroperoxide (tBHP) and the protective effect of probucol on senesecence. Methods Human glomerular mesangial cells(hGMC) were cultured in vitro and intervened by tBHP. The cell survival rate was observed by methyl thiazolyl tetrazolium( MTT). β-gal staining and cell cycle analysis were used to identify cell senescent status;transmission eletric microscopy was used to evaluate the ultra-microstructure of hGMC. Senescent-related indexes were detected after treatment with probucol. Results The cell survival rate with 30 μmol/L tBHP was (80. 12 ± 3. 25 ) % , the positive rate of β-gal staining was significantly higher in tBHP-induced cells (about 81% )than that of the control cells( P <0. 01). 86% of the cells was arrested at G0-G1 phase. Invagination of nucleus membrane and chromatin condensation at the nuclear margin in tBHP-induced cells was observed through transmission eletric microscopy. In the probucol intervented cells, the cell survival rate was higher than that of tBHP-induced cells (92. 68 ± 5.03) % vs. ( 80. 12 ± 3. 25) % (P < 0. 05 ). The positive rate of β-gal staining decreased to 45. 2%. The proportion of cell cycle stage was similar to the control cells.The change of morphous and ultrastructure was relieved. Conclusions tBHP can induce hGMC senescence in vitro and probucol may play a role in preventing hGMC senescence.
