Effect of hemoperfusion with adsorbents containing ser ligand for endotoxin removal on lung injuries in a porcine model of endotoxemia
10.3760/cma.j.issn.1671-0282.2010.10.011
- VernacularTitle:聚偏氟乙烯-丝氨酸新型吸附膜血液灌流对猪脓毒血症肺损伤的影响
- Author:
Qiuping XU
;
Pengfei WANG
;
Ning LI
;
Man HUANG
;
Jianping GAO
- Publication Type:Journal Article
- Keywords:
PVDF;
L-serine;
Hemoperfusion;
Endotoxemia;
Lung injury;
Inflammatory responses;
Oxidative stress;
Pig
- From:
Chinese Journal of Emergency Medicine
2010;19(10):1050-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective A new type adsorbent for removal of bacterial ET was prepared by immobilizing L-serine covalently onto polyvinylidene difluoride membrane (PVDF-Ser) to study the effect of hemoperfusion on sepsis induced lung injuries in a pig model. Method Endotoxemia were induced in thirteen pigs by injecting endotoxin (1 μg/(kg·h) ) intravenously for 2 h. One hour later, the pigs were treated with absorber of PVDF-Ser volumn (group hemoperfusion) or empty adsorber (group control) for 2 h. Extravascular lung water indexed to body weight and alveolar capillary permeability, levels of serum endotoxin, tumor necrosis factor-α, interleukin-6, malondialdehyde, glutathione, myeloperoxidase activity, and lung histological changes and myeloperoxidase immunohistochemical analysis were measured. Results Hemoperfusion treatment with PVDF-Ser volumn significantly improved extravascular lung water, pulmonary alveolar capillary permeability and lung injuries ( P ＜ 0. 05). In addition, hemoperfusion attenuated both serum and pulmonary cytokines, and decreased lung malondialdehyde and myeloperoxidase levels ( P ＜ 0. 05). Conclusions Hemoperfusion with the adsorber of PVDF-Ser volumn effectively reduced inflammatory responses and improved lung injuries and oxidative stress in endotoxemia. It has a high potential of clinical application for treatment of patients with severe sepsis.
