The role of hIL-10-MSCs in immune tolerance of rat discordant liver xenotransplantation
10.3760/cma.j.issn.1007-8118.2010.10.020
- VernacularTitle:hIL-10修饰骨髓间充质干细胞对大鼠原位异种肝移植急性排斥反应的影响
- Author:
Jian NIU
;
Bin LIU
;
Yewei ZHANG
;
Xiangnong LI
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Rejection;
Mesenchymal stem cell;
Immune tolerance
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(10):777-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of hIL-10-MSCs on rejection of rat discordant liver xenotransplantation. Methods The model of cavia to rat discordant liver xenotransplantation was established. The rats were randomized into the control group, MSCs group, and hIL-10-MSCs group.The survival time and liver function of each recipient were observed and expression levels of E-Selectin, LFA-1, VCAM-1, and NF-κB determined by RT-PCR and ELISA 24 h after transplantation.Results The survival time was prolonged, liver function improved, and expressions levels of E-Selectin, LFA-1, VCAM-1 and NF-κB were significantly decreased in the hIL-10-MSCs group (P＜0.05).Conclusion hIL-10-MSCs are able to protect transplanted liver and decrease the expression of E-Selectin, LFA-1, VCAM-1, and NF-κB in the liver.
