Antioxidative effect of schisanhenol on adipokine expression in 3T3-L1 adipocyte
10.3760/cma.j.issn.1008-1372.2010.09.001
- VernacularTitle:五味子酚对3T3-L1细胞氧化应激模型脂肪细胞因子表达的影响
- Author:
Zhenzhong JI
;
Zhe DAI
;
Li NIU
;
Yan HUANG
;
Yancheng XU
- Publication Type:Journal Article
- Keywords:
SCHISANDRA CHINENSIS/PD;
Oxidative stress/DE;
Adipokines/ME
- From:
Journal of Chinese Physician
2010;12(9):1153-1157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of schisanhenol on adipokine expression in 3T3-L1 adipocyte and its related mechanism. Methods 3T3-L1 adipocyte was cultured in vitro and induced to differentiation and maturity. Glucokinase was added to culture medium to make an oxidative model. The expression of adipocytokines were detected under the circumstance of different doses and at different time points of schisanhenol. Results The expression of adiponectin, leptin, resistin and visfatin were decreased with the increase of glucokinase concentration. Concentration-dependent inhibition effect was most obvious in leptin (25 mU/ml Glucokinase vs Blank group, t =7.29, P＜0.01). With pretreatment of oxidative stress, the adipocytokines increased as the doses of schisanhenol increased (t=6.31,P＜0.01 in adiponectin;t=5.92, P＜0.01 in leptin; t=3.77, P＜0.05 in resistin; t=3.63,P＜0.05 in visfatin). With the extension of schisanhenol effect, the expression of four adipokines showed the process of first decrease-then increase'. The effects of schisanhenol on adipokines were parallel with the alteration of oxidative stress. Conclusions Schisanhenol increased adipocytokines expression in 3T3-L1 adipocyte by reducing oxidative stress, and the increase of leptin and adiponectin were most obvious, which indicated that schisanhenol could play a role in the treatment of diabetes by Chinese herb wuweizi.
