Differential expression of serum proteins of pancreatic cancer patients
10.3760/cma.j.issn.1674-1935.2010.04.007
- VernacularTitle:胰腺癌患者血清相关蛋白质差异表达的研究
- Author:
Xinghua LI
;
Dening FANG
;
Yi SHEN
;
Chaiming CENG
;
Shujuan DENG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Proteonics;
Serum proteins;
Tumor markers,biological
- From:
Chinese Journal of Pancreatology
2010;10(4):245-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective Serologic proteome analysis method (SERPA) was used to compare the different of the serum proteins between normal and pancreatic cancer patients' serum, and to find a new specific pancreatic cancer marker. Methods HPLC was used to eliminate albumin from the serum, two-dimensional electrophoresis was used to separate the proteins. After imaging collection and analysis, the different proteins between pancreatic cancer and normal subjects were cut for mass spectrometry. Results Four discrepancy proteins were obtained. Guanylate cyclase-activating protein 2 was highly expressed in normal serum but not pancreatic cancer. Hp2-alpha, transthyretin and KIAA1018 protein were highly expressed in cancer patients'serum but not normal people. Conclusions KIAA1018 may become a promising tumor marker for screening and early diagnosis of pancreatic cancer.
